KC Informatics — MAST Molecular Diagnostics

Raw data to assay intelligence.

Due to confidentiality and proprietary tool development, we cannot show internal workflows publicly. What we can show is the shape of the system: KC Informatics turns multi-genome datasets, target genes, cross-species contrast sets, alignments, differential calls, and assay-development requirements into a guided analysis workspace.

One streamlined tool can replace weeks of manual file handling, comparison, specificity checking, filtering, and reformatting.

Genome data Target gene Cross-species specificity Differential analysis IP-ready outputs
One Shot Analysis
Inputs Multi-genome zip or combined FASTA plus target gene.
Find Target regions extracted with optional flanking sequence.

Mutation-aware alignment

Compare Target-vs-other labelling to expose species-specific signal.
Convert Aligned sequence evidence becomes design-ready matrices.

PWM signal

Mutation-aware alignment

PWM signal

From raw datasets to delivered assays

The public preview uses mock outputs, but the concept is real: raw datasets enter once, and the workspace returns organised evidence along with specificity-aware molecular diagnostic assays.

1place to combine multi-genome data, search, compare, align, inspect, and export.
weeksof manual data processing compressed into repeatable analysis runs.
tailored approachesassay evidence shaped by cross-species differentiation and contrast genomes.

The Workflow

Inputs
multi-genome zipcombined.fatarget genenon-target genomes
Processing
BLAST hitsflanksmutation mapdifferential labellingspecificity screen
Readouts
alignment viewerPWM matrixspecies-split signalexportable evidence
Outcome
delivered assaysmutation-targeted LAMP panelsclient-ready molecular outputs

Organised information

Analysis outputs, project notes, and training material can be presented as a readable workspace instead of scattered files and ad hoc explanations.

Confidentiality note. Internal KC Informatics workflows include novel tooling and proprietary assay-development logic. The restricted portal protects that work while this preview shows the accessible, end-to-end analysis experience built around it.

The same workspace also turns lab notes, intermediate outputs, and decision points into organised web-format records, making results easier to review, present, and use for training others through the workflow.

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Helicobacter pylori

Across this project, each target region is aligned to a reference sequence, variants are called, and resistant isolates are compared with susceptible isolates to identify a practical set of assay targets. The antibiotic-specific workflows then tighten or relax the target-selection rules according to how consistent the resistance signal is for each drug.

The primary datasets used here were a Vietnamese cohort and a phenotyped whole-genome study linked to the PRJNA1242368 dataset. Clarithromycin and tetracycline are handled differently because their principal resistance sites are already well established in the literature, so those sections focus on validated targets and saved primer builds rather than de novo target discovery.

Koala

In koalas, Chlamydia pecorum is a major cause of conjunctivitis, cystitis, reproductive-tract disease and infertility. Koala retrovirus (KoRV) is important alongside it: overall KoRV burden is associated with greater susceptibility to chlamydial disease and a higher risk of progression to chlamydiosis, with the relationship operating at the level of viral load rather than being confined to a single subtype.

The comparative genome set used for this page was procured from NCBI and BV-BRC. The differentiation target chosen here is ompA, using the combined alignment to locate a discriminatory block and the pecorum-only consensus to keep the final primer sequences representative of the intended target population.

Mycoplasma content is still being assembled. For now, the active workflow remains in the H. pylori section.